UHK ∙ Students, Research, International, Employees ∙06/01/2024

Evaluation of chiral SFC-MS for the analysis of positional isomers and enantiomers of biologically significant lipids

Project Description

Most lipids exist in distinct isomeric forms, which are assumed to serve different intracellular functions. They are rarely studied in current lipidomics due to sample complexity, time- and experimentally demanding isomer separation, or lack of standards. The main objective of the project is to evaluate chiral supercritical fluid chromatography with mass spectrometry detection for high-throughput and comprehensive analysis of biologically significant lipids represented by diacylglycerols and fatty acid esters of hydroxy fatty acids, providing a number of distinct positional isomers and enantiomers. This project focuses on multidisciplinary research in synthetic and analytical chemistry with application potential in physiology. The greatest attention will be paid to the synthesis of commercially unavailable enantiomeric standards for qualitative and quantitative analysis and the variation of separation selectivity of lipid classes and positional isomers important in the analysis of real samples. Finally, the composition of isomers in selected biological samples will be determined.